Isolation, Identification and Characterization of Antimicrobial Activity Exhibiting Actinomycete Streptomyces paradoxus Strain KUASN-7 from Soil

Soil acts as a rich source of Actinomycetes, which are generally characterized potent antibiotic producers. The research rationale this study was to isolate and characterize potential Actinomycetes possessing properties from the soils Dharwad Karnataka, India. Further, isolates were using microscopic, biochemical molecular techniques. Initially, ten different isolated on starch casein agar media. Out isolates, five them Gram-positive further screened for antimicrobial potency by cross streak method. Among 5 studied, SN-7 found be most efficient against all four pathogens tested, confirmed its efficacy. active identified Streptomyces strain 16S rRNA gene sequence similarity analysis given no. SN-7. Genebee NEB Cutter version 2.0 online tools used confirm secondary structure restriction sites DNA respectively, deposited with NCBI an accession number KX284898, In addition, subjected preliminary screening bioactive metabolites column chromatography eluted fractions UV FTIR spectroscopy